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Writer's pictureTony Vines

Endoscopic Pancreas Fluid Collection: Methods and Relevance for Clinical Care and Translational Science


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Date:

2016 Sep

PMID:

Category:

2

Authors:

Phil A Hart 1, Mark Topazian 2, Massimo Raimondo 3, Zobeida Cruz-Monserrate 1, William E Fisher 4, Gregory B Lesinski 1, Hanno Steen 5, Darwin L Conwell 1

Abstract:

Pancreatic secretions have an important role in the regulation of a normal nutritional state but can be altered owing to a variety of pathophysiological mechanisms in the context of exocrine pancreatic disease. The development of an endoscopic technique for collection of pancreatic fluid, termed endoscopic pancreatic function testing, has led to improved understanding of these alterations and is particularly helpful to characterize chronic pancreatitis. In addition, investigators have found endoscopically collected pancreatic fluid to be a valuable biofluid for the purposes of translational science. Techniques such as proteomic, cytokine, genetic mutation, DNA methylation, and microRNA analyses, among others, can be utilized to gain a better understanding of the molecular characteristics of chronic pancreatitis and other pancreatic diseases. Endoscopic collection of pancreatic fluid is safe and relatively straightforward, permitting opportunities for longitudinal analysis of these translational markers throughout the course of disease. This manuscript summarizes our current knowledge of pancreatic fluid, with an emphasis on proper techniques for sample collection and handling, its clinical utility, and preliminary observations in translational science.


 

Acknowledgements:

The content of this article is solely the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute, the National Institute of Health, or the National Institute of Diabetes and Digestive and Kidney Diseases.


The Study of Chronic Pancreatitis, Diabetes, and Pancreatic Cancer (CPDPC) Research Consortia is supported and funded by grants from the National Cancer Institute and the National Institute of Diabetes and Digestive and Kidney Diseases under the following award numbers:

Project Number:

Awardee Organization

U01DK108326

Baylor College of Medicine

U01DK108314

Cedars-Sinai Medical Center

U01DK108332

 Indiana University

U01DK108323

Kaiser Foundation Research Institute

U01DK108288

Mayo Clinic

U01DK108327

Ohio State University

U01DK108300

Stanford University

U01DK108320

University of Florida

U01DK108306

University of Pittsburgh

U01DK108328

University of Texas MD Anderson Cancer Center


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